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In basic, most of the cytokines detected in BALF have been considerably increased in WT mice uncovered to PG with nicotine compared to PG alone or air control [42]. Some of these effects had been discovered to be by way of nicotine activation of NF-κB signalling albeit in females however not in males. In addition, PG with nicotine caused increased macrophage and CD4+/CD8+ T-lymphocytes cell counts in BALF compared to air control, but these results have been ameliorated when animals have been sub-chronically uncovered to PG alone [42].